Journal: Cell Reports Methods

Article Title: Phenotypic deconvolution in heterogeneous cancer cell populations using drug-screening data

doi: 10.1016/j.crmeth.2023.100417

Figure Lengend Snippet: Key resources table

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies QuikChange II XL site directed mutagenesis kit Agilent 200521 Biological samples Bone marrow samples derived from patients with multiple myeloma Oslo Myeloma Center N/A Chemicals, peptides, and recombinant proteins Trypsin:EDTA Solution - 0.05% Gemini Bio Products 400–150 DPBS Gibco 14,190–144 RPMI 1640 Corning 10-040-CV L-glutamine Sigma-Aldrich G7513 Penicillin-Streptomycin Gemini Bio Products 400,109 Fetal Bovine Serum Gemini Bio Products 100–500 Erlotinib LC Laboratories E−4007 Propidium Iodide Invitrogen P1304MP Hoechst 33,342 Invitrogen H21492 CellTracker Green CMFDA ThermoFisher C7025 CellTracker Orange CMTMR ThermoFisher C34551 Lymphoprep Stemcell Technologies 04-03-9391103 Dynabeads CD8 ThermoFisher 11147D Dynabeads Human T-activator CD3/CD28 ThermoFisher 11132D Human interleukin-2 Roche 10,799,068,001 CD138 microbeads Milteny Biotec 130-051-301 Dimethyl sulfoxide (DMSO) Sigma-Aldrich D5879 Benzethonium chloride (BzCl) Sigma-Aldrich 843,983 Dexamethasone (Immunosuppressant) Selleckchem S1322 Ixazomib (Proteasome inhibitor) Selleckchem S2180 Thalidomide (Immunomodulatory) Sigma-Aldrich 50-35-1 Selinexor (CRM1 inhibitor) BOC Sciences 1,393,477-72-9 Melflufen (alkylating agent) Oncopeptides N/A Venetoclax (Bcl-2 selective inhibitor) Selleckchem S8048 Imatinib Cayman 13,139 Critical commercial assays CellTiterGlo Promega G7573 Deposited data PhenoPop code repository This paper https://doi.org/10.5281/zenodo.7334379 pyPhenoPop code repository This paper https://doi.org/10.5281/zenodo.7323577 Experimental models: Cell lines HCC827 ATCC CRL-2868 H1975 ATCC CRL-5908 BAF3 Kindly provided by Dr. Kristine Kleivi ACC-300 Oligonucleotides Forward primer for establishing of BCR-Abl-T315I 5′ GGGAGCCCCCGTTCTATATCATCATTGAGTTCATGACCTACG-3′ This paper N/A Reverse primer for establishing of BCR-Abl-T315I 5′ CGTAGGTCATGAACTCAATGATGATATAGAACGGGGGCTCCC-3′ This paper N/A Recombinant DNA pRNAT-H1.1/Hygro-GFP GenScript SD1214 pcDNA3::p210-BCR-Abl Addgene 27,481 Software and algorithms MATLAB version R2020b Mathworks, USA https://www.mathworks.com/products/matlab.html Python version 3.8 Python Software Foundation https://www.python.org PhenoPop code repository This paper https://doi.org/10.5281/zenodo.7334379 pyPhenoPop code repository This paper https://doi.org/10.5281/zenodo.7323577 ImageJ Sage et al. 33 https://imagej.nih.gov/ij/ Other Cellomics Arrayscan High Content Screening Platform Thermo N/A 96-well black bottom plates Corning Inc 3904 384-well TC-plates Greiner Bio-One 781098 Automatic liquid dispenser Fritz Gyger N/A Acoustic dispenser Echo 550 LabCyte N/A Plate reader Perkin Elmer N/A Open in a separate window Key resources table .

Techniques: Mutagenesis, Derivative Assay, Recombinant, Software, High Content Screening